Can we culture THP1 in Minimum Essential Medium instead of the ATCC suggested RPMI?

I have seen some answers saying that we can use DMEM instead of RPMI (11.89mM Glucose). But I want to culture them in a low glucose medium (5mM Glucose) as per my experiments and we don't have low...

06 July 2015 2,333 3 View

Why are THP1 monocytes getting adhered to the dish during serum starvation?

THP1 monocytes, despite of them being a suspension culture are getting adhered (all the cells) to the dish during serum starvation (at least 45mins to 1hr in basal RPMI). They are adhering so...

05 June 2015 6,748 8 View

Can anyone suggest an antibody that can detect CD133 in MCF-7 cell line?

I have used CD133/1 antibody (Human) but didn't observe a detectable amount of the protein in MCF-7 (although I did it only once). There are two possibilities here. 1. There's a mismatch of the...

02 March 2014 636 0 View

What are the effects of the presence of insulin in the culture medium of MCF-7?

As per ATCC guidelines, there should be a 0.01 mg/ml of insulin in the medium. Please explain if there are any noticeable changes (phenotypic and genotypic) in the cell line?

02 March 2014 7,374 0 View

Why do we use nested primers in TAIL PCR?

What is the difference between normal primers and nested primers and why are nested specfically used in TAIL PCR?

04 May 2013 6,269 8 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

Any Custom Peptide Synthesis Companies In India? Custom Peptides Manufacturing Lab in India?

We are looking for many Custom Peptides in India, with good quality for our Bio-pharmaceutical research. Please suggest us best Custom Peptide Synthesis and Service Providers from India? Expecting...

31 December 2020 6,516 7 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View