What is the successful procedure for synthesis core and shell quantum dots like CdSe/ZnS?

I have synthesized CdSe with green emission but the shell of ZnS couldn't formed. The procedure is cadmium oxide and Se dissolved in oliec acid with heating in the presence of paraffin liquid,...

15 September 2020 7,884 2 View

Hot press machine curing time?

Dear All, I am planning to cure (T700GC/134) carbon fiber prepreg, using hot press machine. Could anyone please suggest the curing time, temperature and pressure for me? Your cooperation is much...

17 December 2019 5,719 1 View

What's the proportion for the peak area of identifiable compounds based on NIST database to the total detected products used GC-MS?

25 July 2017 7,830 10 View

How can get the phylogenetic tree?

I have many sequences from the environment to do function gene but I am not experienced analysis sequence to get the phylogenetic tree, please could someone help me step by step how I can do this?...

23 May 2017 8,628 4 View

Any advice on Quartz XRD plate analysis?

18 October 2015 523 5 View

Can any one tell me which solvent nitrile butadiene rubber?

03 November 2014 1,239 5 View

What format file can I use with WinPLOTR?

13 October 2014 1,727 5 View

Children with diabetes and covid19?

I am asking about the relation between covid 19 and diabetes. How can we treat covid infected children who are already suffering from diabetes?

01 January 1970 2,900 1 View

I have two questions; academic and technical?

in terms of the academic inquiry, i am interested in ekphrastic poetry, which is an interdisciplinary genre connecting poetry to art (painting, sculpture, pictures, captions, photography). i am...

01 January 1970 996 3 View

Publication period length?

dear highly esteemed researchers and academicians on the RG platform. is there any plausible and possible way, other than surfing the journal's homepage, in which we can know how long does it take...

01 January 1970 2,792 35 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View