Apart from amplification requirements being mandatory for NGS, what are the other differences or advantages third generation sequencers like Oxford nanopore and PacBio provides? Which one is more suitable for RNA-seq?
If there are any references pertaining to the pros and Cons of the two generations, please elaborate
Warm regards
that is such a complex question that you should really just google some review article on this subject
There is no absolute "NEXT", and after first generation all type of massive parallel sequencing are NGS. If you are assigning the numbers of generations then "next" should not be included in that definition.
Furthermore, the choice of sequencing platform depends on the aim of sequencing and budget. But for RNA-seq, microarray and Illumina sequencing are preferred.
- Badges
- Science method