What are the differences between n-acetyl cysteine and L-cystine mechanism of action?

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Can you suggest reliable procedures to get displacements from accelerations in frequency domain ?

I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.

21 July 2024 3,465 5 View

How can I calculate the mass of hydrogen that left the system?

Hello everyone, I have a question. Assuming I have a 200 kg hydrogen tank at 200 bar, and assuming I can't go below a pressure of 20 bar. How can I calculate the mass of hydrogen that left the system?

06 June 2024 2,216 2 View

How to calculate pressure drop of compressed gases?

Good morning I have a question. How can I calculate the distributed pressure drops for a compressed gases in pipe? For Example, hydrogen compressed at 30 bar with a flow of 195 Nm^3/h. In a UHP...

17 March 2024 5,572 5 View

I want to change my mail address ?

Please note the my new mail address is "[email protected]"

06 March 2024 6,554 0 View

SLoreta: how to visualize a data (ROIxBANDS)?

I have many files ROI regionsXband(84x7). I would like to visualize it on sLoreta but when i convert the file txt into Slor, the output is empty and i can't visualize it. Anyone know how resolve it?

31 January 2024 2,996 0 View

How to get rid of Streptavidin-HRP background/noise on westernblot?

Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...

16 October 2023 3,778 2 View

How to measure area and perimeter of dissected fern leaves?

Dear Colleagues, I have been measuring the area of tropical fern leaves for several species, however the perimeter is a different and difficult task. Ferns have higlhy divided and the small...

06 September 2023 3,680 2 View

How to solve unexpected movement of rigid tools in Abaqus Explicit?

Hello fellow Abaqus users, I've been working with Abaqus for the past two years and have encountered a significant issue in my simulations related to the plastic deformation of sheet metals....

06 September 2023 3,663 4 View

What is the best way to analyze calcium spark recordings in VSMCs?

I'm having a really hard time finding software to help me analyze calcium sparks. I use VSM cells and the fluo-4 am calcium indicator, I record 30s (80 frames) in the LSM780 confocal, each...

02 August 2023 4,094 3 View

Are there any drawback with horizontal hoods for Pharmaceutical industry and CROs?

Hi, I am currently working on a device tailored for the Pharmaceutical industry and CROs. While it is most suited for utilization within a hood with horizontal laminar flow, I have only...

10 May 2023 6,152 2 View

GSH estimation assay: What is the right choice of standard?

Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...

01 August 2024 8,217 1 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

Hi, How can I design primers to obtain Deletion mutants by Site Directed Mutagenesis?

I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...

05 June 2024 9,430 1 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

29 April 2024 3,227 2 View

How to use ORP Combination Electrode for GSH/GSSG Measurments?

Hello, My lab recently acquired a combination ORP electrode, with the refrence being Ag/AgCl and the working electrode being platinum. We are hoping to use this electrode to measure the...

26 April 2024 6,914 1 View

Require single protocol for mice brain (hippocampus) homogenization for ELISA, GSH, and NO assay?

I plan to do ELISA, GSH, and NO assay from mice hippocampus. However, because of limited tissue, I can't homogenise tissue according to different protocols. By doing some literature, below is the...

15 April 2024 8,423 0 View

How to calculate (tot) glutathione peroxidase activity (GPx)?

Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results. I run a...

03 April 2024 9,344 0 View