Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

04 April 2019 3,719 10 View

Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

03 April 2019 2,947 8 View

What would be the best approach to perform a determination of curcumin in mice organs (biodistribution assay) by fluorescence spectroscopy?

07 August 2018 517 2 View

What would be the best approach to perform a determination of curcumin in mice organs (biodistribution assay) by fluorescence spectroscopy?

03 August 2018 898 2 View

What is the best cationic material/compound to stabilize a drug nanocrystal dispersion for subcutaneous delivery?

I've been searching for cationic materials to stabilize a suspension of drug nanocrystals. We want to prepare curcumin nanocrystals (with a bottom up approach, probably) but we need them to be...

03 April 2018 6,811 5 View

What is the effect of the polydispersity (size particle distribution) on the stability of liposomes?

In other words: is there an effect on the stability when comparing highly monodisperse liposomes (for example 100 nm +/- 5) versus highly polydisperse liposomes (for example 100 nm +/- 50 nm? Is...

10 November 2017 6,414 6 View

Can solvents used in histopathology dissolve substances that I need to observe in a specific mice tissue?

01 February 2016 6,487 5 View

Can solvents used in histopathology dissolve substances that I need to observe in a specific mice tissue?

01 February 2016 8,549 4 View

Which are the best methodologies to extract protein from liposomes?

We are trying to quantify protein encapsulated in liposomes. Since lipids in BCA interfere with the measurement, we need to perform a extraction and get rid off the lipids but with a good recovery...

01 January 1970 5,354 5 View

Is cytotoxicity of stearylamine bearing liposomes related to the molecule or to the zeta potential?

I'm preparing cationic liposomes in order to perform in vitro cell assays, so we need to test cytotoxicity first. But I'm not sure if the problem is stearylamine as itself or a high zeta...

01 January 1970 9,415 10 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View