David E. Bautista-Erazo

9 Questions 88 Answers 0 Followers

Questions related from David E. Bautista-Erazo

Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

04 April 2019 3,020 8 View

What would be the best approach to perform a determination of curcumin in mice organs (biodistribution assay) by fluorescence spectroscopy?

Hello. In my laboratory we need to perform the quantification of curcumin in some mice organs after subcutaneous administration of that molecule (“biodistribution” bioanalysis). Our experimental...

08 August 2018 583 2 View

What is the best cationic material/compound to stabilize a drug nanocrystal dispersion for subcutaneous delivery?

I've been searching for cationic materials to stabilize a suspension of drug nanocrystals. We want to prepare curcumin nanocrystals (with a bottom up approach, probably) but we need them to be...

04 April 2018 6,870 5 View

What is the effect of the polydispersity (size particle distribution) on the stability of liposomes?

In other words: is there an effect on the stability when comparing highly monodisperse liposomes (for example 100 nm +/- 5) versus highly polydisperse liposomes (for example 100 nm +/- 50 nm? Is...

11 November 2017 6,489 6 View

Can solvents used in histopathology dissolve substances that I need to observe in a specific mice tissue?

We need to observe a compound (curcumin) in the footpad of a mouse. Solvents used for histopathology are formaldehyde and nitric acid. In addition, we have to decalcify the bone in order to do the...

02 February 2016 6,564 5 View

What are the differences between exosomes and liposomes?

01 January 1970 3,421 2 View

Is cytotoxicity of stearylamine bearing liposomes related to the molecule or to the zeta potential?

I'm preparing cationic liposomes in order to perform in vitro cell assays, so we need to test cytotoxicity first. But I'm not sure if the problem is stearylamine as itself or a high zeta...

01 January 1970 9,492 10 View

Which are the best methodologies to extract protein from liposomes?

We are trying to quantify protein encapsulated in liposomes. Since lipids in BCA interfere with the measurement, we need to perform a extraction and get rid off the lipids but with a good recovery...

01 January 1970 5,430 5 View

What's the regulation of chloroform in pharmaceutical preparations and vaccines? Can you use it in order to create liposomes in a large-scale way?

I am using chloroform in my preparation of liposomes. Is it a big a problem during the scaling up? Thanks.

01 January 1970 8,019 19 View