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Questions related from David E. Bautista-Erazo
Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...
04 April 2019 3,034 8 View
Hello. In my laboratory we need to perform the quantification of curcumin in some mice organs after subcutaneous administration of that molecule (“biodistribution” bioanalysis). Our experimental...
08 August 2018 598 2 View
I've been searching for cationic materials to stabilize a suspension of drug nanocrystals. We want to prepare curcumin nanocrystals (with a bottom up approach, probably) but we need them to be...
04 April 2018 6,882 5 View
In other words: is there an effect on the stability when comparing highly monodisperse liposomes (for example 100 nm +/- 5) versus highly polydisperse liposomes (for example 100 nm +/- 50 nm? Is...
11 November 2017 6,502 6 View
We need to observe a compound (curcumin) in the footpad of a mouse. Solvents used for histopathology are formaldehyde and nitric acid. In addition, we have to decalcify the bone in order to do the...
02 February 2016 6,578 5 View
What are the differences between exosomes and liposomes?
01 January 1970 3,430 2 View
I'm preparing cationic liposomes in order to perform in vitro cell assays, so we need to test cytotoxicity first. But I'm not sure if the problem is stearylamine as itself or a high zeta...
01 January 1970 9,509 10 View
We are trying to quantify protein encapsulated in liposomes. Since lipids in BCA interfere with the measurement, we need to perform a extraction and get rid off the lipids but with a good recovery...
01 January 1970 5,442 5 View
I am using chloroform in my preparation of liposomes. Is it a big a problem during the scaling up? Thanks.
01 January 1970 8,033 19 View