Looking for research papers regarding concrete maturity. Could anyone share with me please?

15 January 2020 8,196 4 View

How to calculate the integration of plastic strain based on plastic strain components (PE) in ABAQUS/Explicit??

Hello, I would like to calculate the plastic strain increment = (max principle strain (3) - min principle strain (1)), which are taken from the plastic strain components (PE) and converted...

24 July 2019 5,296 1 View

How high is the limit of puromycin using in cell selection ?

I am looking for any papers that mentioned about using puromycin in cell selection after transfection. I already got the optimized of MTX and G418. Then I started cell selection with puromycin at...

20 October 2016 6,914 5 View

What is the relationship between Sample concentration and Average count rate in Dynamic Light Scattering measurement ?

When I measure DLS for nanparticles size, I see that there are no rule in the samples concentration and the Average Count rate index in mine. I wonder that if the higher concentration of samples,...

12 January 2015 9,856 9 View

Does anyone work with an existential paradigm in end of life care or palliative care?

I'm looking for colleagues who combine both perspectives as I do in my work for networking and developing mutual research strategies.

07 August 2013 8,691 8 View

Dot Blot protocol

Why do we have to wash the membrane again with TBS only after wash the 3 times with TBS-T ? I I don't wash it again, what happen ?

01 January 1970 7,403 2 View

Densitometer, ImageJ, how to detect the concentration of the band from SDS PAGE ?

After running Elisa, i ran gel and western to confirm the results. I have a question with the densitometer. I have the problem to quantitate the concentration of the protein based on the bands in...

01 January 1970 8,654 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View