Are you talking abt different methods of statistical analysis of mortality data?

Thanks Dear Awais Ihsan

Dear Apurva Kumar

These are ways to estimate the acute toxicity. Actually The most challenging point in these procedures is to use less animals. About Lorke's method this number is 13.

I'm asking about limitations of each procedure

I dont have much details abt finer aspects, but it may be worth looking at the Trimmed Spearmann-Karber method

What is the up and down method which the previous person mentioned? please explain

Thanks Dr Ihsan

Well am also interested in the up and down method for the determination of LD50 but i have read the OECD procedure and am really confused about the statistical analysis and how to get the software. Can somebody help out?

Well, what about cell culture methods? The above mentioned methods seems to be more appropriate for animal studies! Any ideas?

@Awais, ..Thanks, the link was helpful.

Inorder to determine the suitable dose for your invvestigation you should find LD50 using various concentration of that matter and find affectable dose.

Prueba muy utilizada para clasificar la peligrosidad de las sustancias.La utilidad del método depende del efecto que se este buscando y la representación del animal de experimentación. Para el caso reglamentario al confiabilidad la da el Protocolo de método que se utilice, sugiero utilizar los protocolos armonizados especialmente los de u.s.a. o los de la Comunidad Europea que están disponibles en internet

LD50 is more reliable for dosing finding

Thanks Dear Navin & Ali for your comments

Dear Alfonso Would you please write your comments in English

Regards

Recently guidelines are being develop to derive ld50 from lc5o in cell culture of 3t3 filbroblast and normal human keratinocytes. These help in deciding starting dose of the oecd 425 starting dose

i wonder if the method (UDP) can be applied for the fishes since we know this oral administration for the rat/mice.. anyone can suggest me?