Arathi (Sesha) Paluri

3 Questions 21 Answers 0 Followers

Questions related from Arathi (Sesha) Paluri

What would be a simple and quick functional assay for macrophages and B cells?

The idea is show drug of interest is not affecting functional ability of these I'm thinking along the lines of: 1. Stimulate primary macrophages (or just Raw 264.7 cells) with LPS and treat with...

22 October 2020 1,288 3 View

Ultra low attachment (ULA) plates for phagocytosis?

Does anyone have experience working with ULA plates? I plan to do a phagocytosis assay with Raw 264.7 and cancer cell lines (adherent). My goal is flow cytometry I'm wondering about two...

06 March 2020 1,450 6 View

How can I distinguish between ROS that have mitochondrial and non mitochondrial origin?

~10% of the Reactive oxygen species can originate from extramitochondrial sites like NADPH oxidases. Besides knocking out mitochondrial genes, is there a way to determine the differences in cell...

17 September 2014 377 7 View