He guys,
I am working on proteins that are localizing in the cell membrane and matrix. How likely they can interact with transcription factors/proteins localizing in the nucleus?
Thanks i anticipation
The quick and straight-forward answer to your question is, I am afraid, not very helpful!
And it is DIY (do it yourself).
You may have seen a Cell paper last year that revealed zygotic nuclear localization of TCA cycle enzymes. Recently, more and more of metabolic enzymes are being implicated in impacting the chromatin environment. You also know that the cells undergo nuclear membrane disassembly and assembly every cycle, which must allow some degree of promiscuous mixing of proteins between different compartments. So, yes, it is possible that a membrane/matrix protein can interact with nuclear components. The problem is, you have to do the hard work and tell the world that it happens!
Good luck.
Hi Ihteram, if you know the sequence of your protein then you can run it through several online protein identification search engines to see if it has a transcription factor motive.
Thank you so much Anil and Steingrimur. I actually have performed the Yeast two hybrid assy for the target gene and have found that it was interacting with some of the transcription factors. I, however, couldn't confirm the result through BiFC and CoIP. Now that's probably showing that the interaction through yeast hybrid assay was false....
I appreciate your suggestions
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