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Typically, the effect of siRNAs are assayed after 48hrs. During this period, the siRNA is expected to "keep degrading" the existing as well as newly synthesized transcripts. Now, if there is a...
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The last couple years have seen several reports of enhancer RNAs (eRNAs and similar transcripts) that aid enhancer- and activator-dependent transcription. Arguably, a chunk of 5' end of the eRNA...
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For some experiments, I need a control for DNA binding of proteins; and I need a protein that binds DNA sequence-nonspecifically, and, equally importantly, stoichiometrically. Does anyone know of...
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