Anil Panigrahi

6 Questions 78 Answers 0 Followers

Questions related from Anil Panigrahi

Is there an enhancer 'in contact with an active promoter' such that the enhancer itself is NOT transcribing?

Enhancers and target promoters are often found "in contact". Some 'in contact' enhancer-promoter pairs stay dormant, i.e., there is little detectable promoter-driven transcription. But other...

02 October 2017 8,267 5 View

Is it possible to isolate nuclei from mitotic cells?

It is text-book knowledge that somatic cells breakdown their nuclei during mitosis. Yet, a recent report in PNAS (Liu et al.; http://www.pnas.org/content/114/13/3473) claims to have conducted...

27 September 2017 3,798 2 View

How long does it take to degrade target transcripts by siRNA?

Typically, the effect of siRNAs are assayed after 48hrs. During this period, the siRNA is expected to "keep degrading" the existing as well as newly synthesized transcripts. Now, if there is a...

17 December 2014 5,172 4 View

Do eRNAs remain attached with the template DNA or are detached and float around?

The last couple years have seen several reports of enhancer RNAs (eRNAs and similar transcripts) that aid enhancer- and activator-dependent transcription. Arguably, a chunk of 5' end of the eRNA...

27 August 2014 2,668 4 View

Is there a protein that binds DNA stoichiometrically and sequence-nonspecifically?

For some experiments, I need a control for DNA binding of proteins; and I need a protein that binds DNA sequence-nonspecifically, and, equally importantly, stoichiometrically. Does anyone know of...

22 January 2014 2,800 5 View

Is there a way to separate human cells from mouse cells starting with a mixed cell population?

I am wondering if anyone knows of a way to separate human cells from mouse cells in a mixed population. There are some cell surface markers specific for mouse or human cells and may perhaps be...

06 September 2013 6,713 5 View