Hello
I am working on CD19-directed CAR T-cells and using CD19 expressing cell lines for cytokine and killing assays. I want to include different cell lines that have different levels of antigen expression i.e. high, intermediate, low and none. Most of cell lines I have stained were highly positive for example Granta-519, Nalm-6, SUDHL-4, RAJI and T2. I looked online for Daudi but it seems to be also high. I have H929 as a negative.
Can anyone suggest cells that express intermediate and low levels of CD19 ?
Thanks
Abdulrahman