7 Questions 5 Answers 0 Followers
Questions related from Abdulrahman Bahashwan
When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...
09 November 2019 796 3 View
Hello I have a plasmid to transduce on T-cells. The sequence of this plasmid contains OM-1, which I know only it helps for protein expression, but how does it work I couldn't find an explanation...
03 September 2018 4,598 1 View
Hello, I work in cancer immunotherapy particularly in CAR T-cells. I have leukaemia cell lines such as KO-52, ksaumi and THP-1. They express my target antigen at different levels. My CARs are also...
20 June 2018 1,709 2 View
Hello there, I'm doing protein concentration for WB assay using BioRad DC™ Protein Assay similar principle to Lowry assay. I have 8 samples and standard.I do them in triplicates. However, I've...
11 May 2018 6,507 8 View
Hello I have AML cells that have been cultured on stromal cells for weeks and would like to do flow staining looking for a specific antigen in the AML cells. I am not sure where these cells came...
29 January 2018 4,904 2 View
Hello I am working on CD19-directed CAR T-cells and using CD19 expressing cell lines for cytokine and killing assays. I want to include different cell lines that have different levels of antigen...
16 June 2017 704 2 View
HelloI did restriction digestion and ligation of a huamn gDNA construct and I have now the pure plasmid DNA and want to check the sequence alignment.I've read the things that should be considered...
12 September 2016 1,075 3 View
