Combustion analyzers are generally used to measure TOC, it is a specialized piece of equipment that costs a good amount of money. If there are any 3rd party Environmental testing labs close to you, it is not very expensive to get tested, less than 100 USD. If you have a spectrophotometer, you could probably use the Folin-Ciocalteau method to measure phenolic compounds.

Dear Jonathan

Thank you for your answer.

Can you help me to get the steps for the Folin-Ciocalteau method?

http://www.sciencedirect.com/science/article/pii/S1658365513000514

Above is a link to an article on Folin and GCMS analysis of total phenolics in vegetable matter. The attachment is a more detailed procedure for the Folin method in juice, which would be more applicable to your matrix.

As Jonathan said..Folin ciocalteau method is the best method of determining total phenolic content in any of the source.. UV spectrophotometer is sufficient to analyse. But you need a reference standard like gallic acid for estimation and calculation.

many thanks for you all

Folin reagent is a typically used sum parameter for tpc determination. Nevertheless you have to remember that the reagent is well-known to react also with other substances than phenolic compounds (e.g metals, lipids etc.). Furthermore, the tpc you measure depends on the equivalent phenolic compounds which you take for your uv-vis calibration curve. You can take a look at my paper about the effect of omw where we assessed those effects.

Greetings

Christian

Folin ciocalteau method is the best method of determining total phenolic content in OMWW

Yes , Folin ciocalteau method is good method