What are the best specific primers for identification of a lot of Trichoderma isolates at the species level?

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Ganapati Bhat

I don't think there is any specific primer for each Trichoderma Species. However, you can use ITS or translation elongation factor 1- α conserved primers and sequence the amplified segment. Then use the ISTH site to BLAST the sequence and find out the exact species.

ISTH Website

http://isth.info/

Peter Elias Kidibule

ITS and TEF1 should work perfect.

Hassan Nima

this link is useful

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC101429/

regards