How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How post-hoc test should I perform for two way repeated measures ANOVA for 3 groups?

Hello everyone, I have 3 groups and 4 measurements so, I performed two-way repeated measures ANOVA. How can I detect the difference (which groups and which measurements) when there is significant...

27 October 2020 4,503 3 View

How do We Maintain Appropriate Cell microenvironment with 3D Cell Culture Method?

3D Cell Culture is newly optimized and promising discovery in cell organization research and other researches that needs evindences in cell culture (in vitro) level. Are 3D Cell Cultures mimic...

23 September 2020 7,109 4 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

Can you give information about how to calculate the molarity of MEA?

Hi, I am trying to produce CZTS films by sol-gel spin technique. Can you give information about how to calculate the molarity of MEA? Did you calculate the MEA molarity separately for each metal...

11 July 2020 3,415 1 View

Do weak contribution equations within a module use the module's boundary condition in COMSOL?

I am trying to solve a partial wave equation for scattered quasi-TE waves. I found the COMSOL built-in Helmholtz equation module very close to my problem. The bottleneck was that, I couldn't...

03 June 2020 8,937 1 View

How can I solve the high impedance problem of saturated calomel reference electrode?

The reference electrod I use is saturated calomel electrode (3M KCl as the filling solution). I got overload problem while measuring the EIS, so then I checked the impedance of the electrod and...

27 May 2020 7,557 12 View

How Can I find a reverse primer with good rating, if I need to amplify entire gene?

I need to amplify the KCNA1 gene. I could not design the reverse primer with GC content ~50%, G/C content between 40-60%, rating 100, and no secondary structure. Any help much appreciated!

25 May 2020 7,276 9 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

How should I compare pre test and post test of three groups?

Hi everyone, I have 2 treatment groups and 1 control group. I measured some physical parameters as a pre-test and post-test. Should I compare 3 groups pre-test and post-test separately by using...

23 March 2020 8,599 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View