How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How post-hoc test should I perform for two way repeated measures ANOVA for 3 groups?

Hello everyone, I have 3 groups and 4 measurements so, I performed two-way repeated measures ANOVA. How can I detect the difference (which groups and which measurements) when there is significant...

27 October 2020 4,503 3 View

How do We Maintain Appropriate Cell microenvironment with 3D Cell Culture Method?

3D Cell Culture is newly optimized and promising discovery in cell organization research and other researches that needs evindences in cell culture (in vitro) level. Are 3D Cell Cultures mimic...

23 September 2020 7,109 4 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

Can you give information about how to calculate the molarity of MEA?

Hi, I am trying to produce CZTS films by sol-gel spin technique. Can you give information about how to calculate the molarity of MEA? Did you calculate the MEA molarity separately for each metal...

11 July 2020 3,415 1 View

Do weak contribution equations within a module use the module's boundary condition in COMSOL?

I am trying to solve a partial wave equation for scattered quasi-TE waves. I found the COMSOL built-in Helmholtz equation module very close to my problem. The bottleneck was that, I couldn't...

03 June 2020 8,937 1 View

How Can I find a reverse primer with good rating, if I need to amplify entire gene?

I need to amplify the KCNA1 gene. I could not design the reverse primer with GC content ~50%, G/C content between 40-60%, rating 100, and no secondary structure. Any help much appreciated!

25 May 2020 7,276 9 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

How should I compare pre test and post test of three groups?

Hi everyone, I have 2 treatment groups and 1 control group. I measured some physical parameters as a pre-test and post-test. Should I compare 3 groups pre-test and post-test separately by using...

23 March 2020 8,599 8 View

Plasmonics as a collective oscillation of holes instead of electrons?

In metals, the outermost conduction electrons (electron plasma) interact with incident photons and according to the classical Drude model, they may resonate and build up longitudinal plasmonic...

17 January 2020 1,559 7 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View