Would anyone be able to provide me with the book with the title Globalization: A Very Short Introduction?

In view of the research question I posted recently, this book will be of great importance, however, I can't get the soft copy so I was hoping if anyone happens to have it and would really...

24 December 2020 7,199 3 View

How has Globalization affected development in the Global South?

I am a student working on a long essay with my topic as "Globalization; an additional problem or a solution to the problems of the global South countries". In this paper, I seek to discuss the...

26 October 2020 3,966 4 View

Integration of Music - Musical Activities, Drama, Movement and Fine Arts in Elementary Mathematics Teaching?

Does it make sense to discuss this? Learning through and with art? Can an imaginative teaching model be the solution to multiple forms of learning and divergent production? In my doctoral thesis,...

01 December 2019 7,529 3 View

What is the most effective method of assessing a vocational training MCQ?

Hello research experts, I'm currently working on a project that involves assessing the validity/reliability of a four-option MCQ designed to assess trainee performance as well as a training tool....

28 October 2017 6,681 1 View

I need CSMP III. Can anyone help me with that?

it is a model that is used by environmentalists and soil scientists in contaminant transport in soil

30 June 2016 5,562 2 View

Predefined field problem in Abaqus?

22 March 2016 2,617 7 View

I need assistance on aspergillus fumigatus

I am working on aspergillus fumigatus and i want my fellows to help how to isolate and characterize it from four soil samples from different areas

02 January 2012 5,723 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View