I am working on generating pericytes and SMCs but since both have common origin it is difficult to distinguish between the two lineage best on markers. Does anyone know of very specific markers for each of the lineage?
Pericytes are specialized mesodermal cells which line the blood vessels. Besides the early mesoderm marker CD31 for pericytes, there is a helpful chart in the millipore site below that will show you the different markers that will help you identify the more mature smooth muscle cells may be using Desmin. I would suggest you may also check out this histology site which may of help to identify the cell morphology and location. Also, try the BD Biosciences site for selection of these markers. Hope these will be of help to you.
http://www.millipore.com/techpublications/tech2/pericyte
http://www.histology.leeds.ac.uk/tissue_types/connective/connective_cells.php
This ref. may be of help, study done on rat retina in vivo
http://www.iovs.org/content/45/8/2795.long
I would go for double staining: NG2 with DAB + CD31 with permanent red. Then you see pericytes by NG2 and confirm them by morphology by seing blood vassels stained by CD31.
Cheers
The best marker is the gamma glutamyl peptidase activity, very high in pericytes compared to SMCs
ses Vandehaute et al.2011 Current Neurovascular Resarch 8, 258-269
aSMA is not suitable, because pericytes are also positive .... I would take SMMHC (smooth muscle myosin heavy chain) for the SMCs
PDGFR-beta and NG2 I would use to show the difference in cell lineage between PC and SMC.
Pericytes are specialized mesodermal cells which line the blood vessels. Besides the early mesoderm marker CD31 for pericytes, there is a helpful chart in the millipore site below that will show you the different markers that will help you identify the more mature smooth muscle cells may be using Desmin. I would suggest you may also check out this histology site which may of help to identify the cell morphology and location. Also, try the BD Biosciences site for selection of these markers. Hope these will be of help to you.
http://www.millipore.com/techpublications/tech2/pericyte
http://www.histology.leeds.ac.uk/tissue_types/connective/connective_cells.php
This ref. may be of help, study done on rat retina in vivo
http://www.iovs.org/content/45/8/2795.long
I would like to share this paper with the histologic, immunohistochemical, and ultrastructural features of 12 canine perivascular wall tumors of uncertain classification are boarded.
Actin (SMA) or SMMS1 will mark smooth muscle cells almost exclusively in your context.
Pericytes are PDGFRβ+ CD146+ NG2+
http://online.liebertpub.com/doi/abs/10.1089/scd.2012.0647
I would go for double staining NG2 for pericytes and blood vassels stained by CD31, Immunohistochemical (IHC) and double staining (DIF).
I am always trying to find a stable marker for pericytes in the BBB. Joan Abbott and others have published several in this area. You may want to look at her work. Sen gave you an excellent paper to start.
PDGFR beta would be marker for pericyte but not PDGFR alpha.. if i am not wrong.
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