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Has anybody experience in differentiation/polarization of monocytes into macrophages on uncoated glass coverslips (ibidi 8 well chamber, removable)?
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CD14 positive monocytes were needed for transmigration experiments, thus no CD14 positive selection is possible due to activation by this method.
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Cell system: monocyte-derived dendritic cells
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I would like to use protein G dynabeads.
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