I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T cells from the activated ones?
What is the best strategy to differentiate between CD11b+Gr-1+ Neutrophils and CD11b+Gr-1+ Myeloid derived suppressor cells (MDSCs). Since the markers are same for both, how can we differentiate them by FACS?