I am trying to detect molecules that are connected with sugar.
The device is HRMS with ESI and APCI ionization source, but none can do the job.
Is it possible only with MALDI? or are there specific conditions that can help me?
Thanks a lot.
It sounds surprising to me that ESI does not produce a signal with a substance that has hydroxyl groups. Have you tried changing the pH value of your solution?
Dear Itay,
do you see no signals at all, or is the identification/quantification of sugars connected to peptides the problem (e.g. in a software) ? Do you have a chromatography in front or do you only want to analyse single proteins and thus have a direct injection setup?
Noel W Davies Britta Eggers Jürgen Weippert
Thank you for the repond.
Actually the compound is already positive (see file attached).
The main peak is also in the attached file.
Noel W Davies
The problem is that I didn't get 699.3 amu, but 719.3 amu.
Noel W Davies
Thank you, I will try to do it. probably next week.
And I will update.
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