First off, I'm currently working on a trial aiming to attest to a microalgae-bacteria consortium capability to remove selected endocrine disruptors from synthetic wastewater. The tests are going to be held in 250mL erlenmeyers under various conditions.
One of the experiment controls requires stable pH conditions, ideally close to neutrality. I've searched the literature in order to find the best buffering solutions/chemicals/set-ups in this case, but the results were really mixed.
Tris-(hydroxymethyl)-amino methane (TRIS buffer) seems to be the one usually applied in this kind of study, but apparently there's some controversy to its usage (Article Effect of Tris-(hydroxymethyl)-amino methane on microalgae b...
). HEPE seems to be a good alternative too, but I've found no backing information on this type of research...Any opinions on this matter? Thanks in advance!
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