What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

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Alexandra Johnson

Assuming your gene is protein coding:

Removing the IVS will probably lower the expression of your protein.

Deleting the IRES could cause major problems, it's there so two different proteins can be translated from a single mRNA. This could result in cells not expressing the selection marker if expression of both your gene of interest and the marker are driven by the same promoter.

Here's some articles:

Article Bicistronic Vector for the Creation of Stable Mammalian Cell...

Article Intervening Sequences Increase Efficiency of RNA 3' Processi...