To dilute 100% dimethyl sulfoxide (DMSO) to a concentration of 0.1%, you will need to prepare a dilution using an appropriate solvent. Since 100% DMSO is highly concentrated and can be toxic to cells at high concentrations, dilution is necessary for compatibility with cell culture experiments. One commonly used solvent for diluting DMSO is cell culture media, such as Dulbecco's Modified Eagle Medium (DMEM) or Roswell Park Memorial Institute (RPMI) 1640 medium.

Here's how you can prepare a 0.1% DMSO solution:

It's important to note that DMSO is hygroscopic and can absorb water from the environment, so it should be stored in a tightly sealed container and protected from moisture. Additionally, always handle DMSO with caution, as it can readily penetrate skin and may cause irritation or other adverse effects.

For testing plant extracts on cancer cells, you would then prepare your plant extract in an appropriate solvent (e.g., ethanol, methanol, or water) and add it to the cell culture medium containing the diluted DMSO to achieve the desired concentration for your experiments.

Thank you Dr. Arvind Kumar Shukla

But I dissolved the extract in 100% DMSO before diluting it, how can I control between the DMSO concentration and keep it 0.1% and get the desired concentration of the plant because the final concentration (stoke) I got was 600 mg/1 ml of 100% DMSO, in contrast to that. I will start my work with the first concentration of 250 mg and start reducing the concentrations for the plant extract .

Best regards,

To achieve a final concentration of 0.1% DMSO while maintaining the desired concentration of the plant extract, you can follow these steps:

Here's a summary of the steps:

• Calculate V1 using the desired final DMSO concentration.
• Use the dilution formula to find the volume of stock solution (V1) needed.
• Prepare the final solution by diluting the calculated volume of stock solution with DMSO to achieve the desired concentration.

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To achieve a final concentration of 0.1% DMSO while maintaining the desired concentration of the plant extract, you'll need to perform a dilution calculation. Here's how you can do it:

Let's denote:

• C1 = initial concentration of plant extract (600 mg/1 ml)
• V1 = volume of plant extract solution (1 ml)
• C2 = desired final concentration of plant extract (let's say you want X mg/ml)
• V2 = final volume of solution after dilution (let's say you want Y ml)

First, calculate the amount of plant extract needed for the desired final concentration: C1 * V1 = C2 * V2

Now, solve for V2: V2 = (C1 * V1) / C2

Substitute the values: V2 = (600 mg/1 ml * 1 ml) / (X mg/ml)

Now, let's say you want a final volume of 10 ml (Y = 10 ml), then: V2 = (600 mg/1 ml * 1 ml) / (X mg/ml) = 10 ml

Solve for X: X = (600 mg/1 ml * 1 ml) / 10 ml = 60 mg/ml

This means you need a final concentration of 60 mg/ml of the plant extract.

Next, calculate the volume of 100% DMSO needed to achieve the desired final concentration of 0.1% DMSO in the 10 ml solution: 0.1% of 10 ml = 0.001 * 10 ml = 0.01 ml

Now, subtract this volume from the total volume to find the volume of the plant extract solution needed: Volume of plant extract solution = 10 ml - 0.01 ml = 9.99 ml

Therefore, to prepare your final solution:

• Take 9.99 ml of the plant extract solution (600 mg/ml) and add it to a container.
• Then, add 0.01 ml of 100% DMSO to the same container.
• Mix well to ensure uniform distribution.

This will give you a final solution with 0.1% DMSO and a plant extract concentration of 60 mg/ml. Adjust the volumes accordingly if you need a different final volume or concentration.

Hello Hadeel hussein Abuharb

I feel you should reduce the concentration of your plant extract. You will never obtain 0.1% DMSO for 250mg concentration from 600mg/ml (stock) having 100% DMSO.

You must dilute your stock 600mg/ml (100% DMSO) 1000 times with culture media to obtain 0.6mg/ml working solution having 0.1% DMSO.

So, you begin with 0.6mg/ml (600ug/ml) as the first concentration and you may go down up to 50ug/ml.

Best.

Use media devoid of serum for dilution

Arvind Kumar Shukla thank you very much Dr for helping me .

Hi Hadeel hussein Abuharb,

DMSO can be freely miscible with water, ethanol, acetone, acetaldehyde, pyridine, ethyl acetate, dibutyl phthalate, dioxane, and aromatic compounds. You can choose according to the experimental conditions. And you may use the web tool to calculate

https://www.alfa-chemistry.com/solution-dilution-calculator.htm

https://www.alfa-chemistry.com/mass-molarity-calculator.htm