Hadeel Khalouf

5 Questions 9 Answers 0 Followers

Questions related from Hadeel Khalouf

Can one do ELISA on adherent cells cultured on 96-well plate?

Hello researchers ! after searching in literature and not finding a clear answer, hopefully I will find one here. so I want to detect an intercellular protein in adherent cells cultured on...

27 December 2021 7,818 3 View

How can I replace a fluorophore, cloned within a gene, by another one when the restriction sites are not unique ?

Hello researchers ! so I have a gene with a GFP fluorophore that was cloned inside of it with BamH1 restriction sites. I wanted to replace GFP with mCherry but the thing is this enzyme site is not...

20 July 2021 4,071 14 View

What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

27 January 2021 7,870 2 View

Could someone recommend me a successful staining method for detecting iron in adherent mammalian cells?

Hello researchers ! I am new at dealing with mammalian cells. I have now an adherent cell line which I need to detect iron content in them. could someone recommend me a successful staining method...

08 November 2020 7,230 3 View

I have a problem with qPCR and cDNA synthesis, can you help me with a useful protocol?

Hello scientists! I'm working on gene expression stuff in rice plant, and I'm facing a little problem, which is that the starting quantity ,and expression of course,is so low on the qPCR n all...

16 March 2018 4,598 3 View