Hadeel Khalouf

4 Questions 8 Answers 0 Followers

Questions related from Hadeel Khalouf

Can one do ELISA on adherent cells cultured on 96-well plate?

Hello researchers ! after searching in literature and not finding a clear answer, hopefully I will find one here. so I want to detect an intercellular protein in adherent cells cultured on...

27 December 2021 7,773 3 View

How can I replace a fluorophore, cloned within a gene, by another one when the restriction sites are not unique ?

Hello researchers ! so I have a gene with a GFP fluorophore that was cloned inside of it with BamH1 restriction sites. I wanted to replace GFP with mCherry but the thing is this enzyme site is not...

20 July 2021 3,971 14 View

What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

27 January 2021 7,787 2 View

I have a problem with qPCR and cDNA synthesis, can you help me with a useful protocol?

Hello scientists! I'm working on gene expression stuff in rice plant, and I'm facing a little problem, which is that the starting quantity ,and expression of course,is so low on the qPCR n all...

16 March 2018 4,562 3 View