What are some recent publications showing the predictor power of resting state brain acitivity for tasks done outside the MRI scanner?

Looking for recent articles (2018-2020) that showed resting state brain activity can predict cognitive task performance (e.g., attentional and executive functioning tasks) done in the lab AFTER...

09 January 2021 2,310 2 View

Function of SDS in Northern Blot washing step?

In Northern blot, we normally use either 2*SSC+0.1% SDS or 0.1*SSC+0.1% SDS in washing step. Does anyone know what's the function of the SDS in the washing buffer? Thank you in advance

26 November 2020 2,432 1 View

What is the lowest sample size for a fMRI study on the default mode network (DMN)?

I'm interested to start a fMRI study focusing on a group of professionals with special expertise. It's very difficult to recruit them. Just interested to about the smallest sample size possible...

19 November 2020 7,149 2 View

What is best method for computing mean RT of correct trials without info about RTs from incorrect trials?

The psychometric test I have presents 20 test trials to each subject. It does not record the response time (RT) taken toe complete each trial and all I have are the total times taken to complete...

07 November 2020 8,368 2 View

How could I go to the same spot in mouse brain under 2-photon scope every time when I observe the brain through a cranial window?

I have labeled the specific neuronal protein through AAV injection and want to frequently observe the dynamic change of the same cell. I need go to the same spot every time. Could you please give...

24 February 2020 9,799 3 View

Why macrophage M1 and M2 marker both decrease?

Hello all I try to explore the effect of one antibody on macrophage polarization. I find the antibody both decrease the M1 marker and M2 marker. I don't know how to explain this. Anyone of you...

12 September 2019 1,273 3 View

What are some recent spatial navigation studies that examined the differences between attention and perception?

Particularly with respect to distinct neural correlates of attentional and perceptual processes

26 January 2019 2,548 8 View

Job autonomy and Happiness?

In my current research, i am trying to figure out the relationship between job autonomy(independent variable )and happiness in workplace (dependent variable). while designing my questionnaire i...

13 November 2018 1,964 4 View

When performing post-hoc t-tests for a 2 x 2 ANOVA, is it absolutely mandatory to adjust the p-value to 0.025?

I recalled that it's all right to maintain the alpha at .05 without Bonferroni correction. Is Bonferroni adjustment absolutely mandatory to examine the simple main effects. For instance, for a 2...

07 August 2018 3,307 6 View

What are some notable papers on sex differences in optic flow perception?

Some suggestions of current empirical and review papers would help greatly - and of any variables that moderates the main sex effect.

18 June 2018 2,060 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View