searching for sources of enzymatic assays after obtaining them recombinantly
Hi Mustafa Salman . There is a report on RG about assaying chymosin.
Technical Report Assay of Chymosin
These articles can be good starting points. All the best
1. Words of Advice: Teaching enzyme kinetics
https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15537
2. Resurrecting the phoenix: when an assay fails
https://onlinelibrary.wiley.com/doi/abs/10.1002/med.21670
3. Explicit treatment of non Michaelis Menten kinetics in early drug discovery
https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cmdc.202000791
Chymosin is also known as rennin and it is a protein-digesting enzyme that curdles milk by transforming caseinogen into insoluble casein. For the determination of rennin/chymosin enzyme activity, this should be helpful
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