May corruption be good for business and society?

There has been a debate on corruption (whether it is good or bad) for decades.

08 September 2015 4,287 39 View

What is more important for any relation to sustain- 'Love' or 'Mutual Trust'?

Both trust and love are important; however, commitment is given more importance in making any relation successful.

08 September 2015 6,745 34 View

What is the future of Engineering Education in India?

07 August 2015 1,514 12 View

What is the ultimate aim of life?

07 August 2015 1,955 69 View

Do above average minds discuss ideas?

07 August 2015 3,146 67 View

Does a researcher remain alive in the form of research work and citations even after passing away?

Good quality work is always remembered and cited even after death of valuable researchers. They are quoted time to time for their contributions.

06 July 2015 3,326 84 View

What are the ways to keep track of physical movement of inventory?

Please share for further discussions their comparative advantages and limitations.

05 June 2015 7,053 9 View

Does religion drive towards sustainability?

05 June 2015 392 96 View

How is 'Technology Transfer' different/similar from/to 'Technology Management'?

04 May 2015 798 10 View

How important is joint family culture for growth of individual, nation and world?

Many problems such as: Stress, insecurity and other psychological disorders are troubling more now a days. Here is an attempt to investigate these issues from: Nuclear Family Culture...

04 May 2015 2,628 14 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View