I want to do HPLC-SEC that characterizes the low-order oligomers for the low molecular weight peptide Glucagon(Mol.Wt.=3.48kDa). Does anyone know suitable reference peptides/proteins that could build a calibration curve in molecular weight range of say 3kDa up to about 14kDa? The pH of the buffer I am using is pH=3.
I would use:
Insulin chain A (2.5 kDa)
Insulin chain B (3.5 kDa)
Aprotinin or insulin (6.5 kDa or 5.7 kDa)
Ribonuclease A (13.7 kDa)
That's great Omar. That's helpful. Those 2 peptides could help me with my calibration curve; and they are not too expensive to buy!
I would also recomend Ribonuclease A (13.6 kDa, Sigma R-5000), Cytochrome C (12.4 kDa, Sigma C2506) and Lysozyme (14.3 kDa, Sigma L-6876) as they are in your required mass range and very cheap to buy.
As I recall the MW of the pituitary hormone Oxytocin is 1007 Daltons or 1.007 kDa.
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