Gotta love auto-correct!
HILIC covers several types of stationary phases including bare silica, amine, diol, and others. I saw an article claiming cyano was run as HILIC although my experience seems to run it as reverse phase as aqueous systems.
Amine and diol generally run as normal phase with organic solvents, may run as normal phase from 0 to 50% water, and generally run as reverse phase with more than 50% water.
Something interesting that can be done with silica based C18, but not styrene divinyl benzene, is non-aqueous reverse phase where one uses methanol or acetonitrile instead of water, and a less polar solvent to elute the compound. The polymeric reverse phases swell in these solvents and generate high back pressure.
The links below are some of my work for flash columns, but should apply also to HPLC.
http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/Diol_Columns-Pretend_They're_Normal_Phase.pdf
http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/Method_Development_Strategies_for_Amine_Columns.pdf
http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/Strategies_for_the_Flash_Purification_of_Highly_Polar_Compounds.pdf
http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN86_Chimeric_Diol_Column_Behavior.pdf
http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN78_HILIC_Purification_Strategies_for_Flash_Chromatography.pdf
http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN84_RediSep_Rf_Gold_Silica_and_Highly_Polar_Solvents.pdf
http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/Facile_Isolation_of_Carotenoid_Antioxidants.pdf
http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN92_Purification%20of%20C60%20Fullerene.pdf
http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN58_Non-aqueous_Reverse_Phase_with_RediSep_Rf_Gold_C18.pdf
Thanks Jack. That autocorrect destroyed my question for a minute there, but I'm glad you understood. Does ISCO sell any HPLC columns?
Teledyne ISCO doesn't sell HPLC columns anymore. I'd look to Waters and Agilent first. They have many application notes too, though I haven't seen any method development app notes- the links above partially fill that hole.
Hypercarb column from Thermo Fisher Scientific. is graphite carbon based column having unique separation, but this column takes long time to equilibrium. you can find application from the following paper: Journal of Pharmaceutical and Biomedical Analysis 86 (2013) 198–203.
There is a review article from my group -that may help you- dealing with this question and other HILIC parameter:
G. Greco and T. Letzel: Main interactions and influences of the chromatographic parameters in HILIC separations.
Journal of Chromatographic Science, 2013, 51 (7), 684-693.
and there are several HILIC tipps at
Separation Sciences, 2013, http://www.sepscience.com/Techniques/LC
HILIC Solution Series No.3 deals with stationary phases.
Success,
Thomas
The most unique phases for HILIC-like applications are silica hydride. A recent review is in TRAC
I agree with Professor Pesek reply realted to the silica hydride and his recommendation to check the review published in TrAC .
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