How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

12 November 2020 5,568 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

25 October 2020 8,601 6 View

How to handle the analysis of yield stability or yield risk based on simulated crop yield data?

Dear everyone, do you have any experience on the analysis of yield stability or yield risk based on simulated crop yield data? I would like to use a modelling approach and simulate grain yields...

23 February 2020 1,584 6 View

What is the best way to calculate the yield of an entire crop rotation including different kind of crops ?

What is the best way to calculate the yield of an entire crop rotation including different kind of crops and different yield levels ? For example the following cropping sequences: 1. sugar beet...

03 December 2019 9,527 11 View

Protocol for whole heart tissue lysis for Westerns?

Hello, I am troubleshooting a western protocol using whole heart homogenates but I seem to be getting consistent smearing at the lower band range of my gel which is also where I want to see my...

01 October 2019 8,682 4 View

What is the minimum sample size for factor analysis?

I will be doing ryff's psychological well-being scale for psychometric properties. What would be the minimum sample size for factor analysis using spss? I read Slovin's formula is not reliable?

26 April 2017 601 7 View

Could alternate form reliability be administered simultaneously?

I will be doing ryff's psychological well being scale's psychometric properties. Could I administer alternate form reliability simultaneously? (42-item and 18-item)

25 April 2017 8,184 3 View

IS Confrimatory Factor Analysis CFA and WLS enough for psychometric evaluation?

Im going to do a psychometric evaluation on ryff's scale using nurses as my sample. Would CFA and WLS suffice? Thank you!

13 April 2017 4,938 3 View

Can Zung's Depression Scale be administered by a student?

I am currently doing my psychometric evaluation on ryff's scale and would like to use Zung's scale for my divergent validity. I am an undergrad student. Can I administer it?

09 April 2017 510 5 View

What would be a good discriminant validity for Ryff's PWB?

04 April 2017 5,633 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View