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What measures can be done to reduce the increase in LDH activity in serum and heparin plasma samples due to underfilled vacuum blood collection tubes?

This question is written in relation to the article written by Neuwinger, Nick et al. entitled “Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in...

12 April 2024 371 0 View

How does the Sysmex DI-60 digital morphology analyzer help evaluate the shape of blood cells, and what insights does it provide on time for analysis?

This question is asked in relation to the article, "Red and white blood cell morphology characterization and hands-on time analysis by the digital cell imaging analyzer DI-60". The mentioned study...

11 April 2024 4,094 1 View

How to subset large arrays in R?

Hello everyone, I used the his2arr function in Rstudio to create a large array dataset. I tried to subset this array in order to be able to work with the data I need. To do this I used the...

13 February 2022 1,826 6 View

How should I go about doing IHC on mice brains cut at 20 micrometers that are already mounted?

I am looking at staining mouse brain tissue for three different proteins (TPH-1, FFAR 2, and IL-6). The tissue is fixed (24 hours in paraformaldehyde twice, and then 2 rounds of 24 hours in...

29 October 2021 9,435 3 View

How to increase yield for thioglycollate elicited peritoneal macrophages?

Hello, I am learning how to isolate thioglcollate primed peritoneal macrophages from mice but my yield has been lower than expected. I expect a total yield of 1x10^7 but I have had inconsistent...

22 February 2021 2,200 4 View

How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

12 November 2020 5,838 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

25 October 2020 8,885 6 View

How do I construct the electric fields of different light sources?

Hi all, I'm trying to do some basic propagation of light sources through an optical scheme, however, I'm not sure how to define the electric field for the different sources. I want to start with...

02 April 2020 5,219 0 View

How to handle the analysis of yield stability or yield risk based on simulated crop yield data?

Dear everyone, do you have any experience on the analysis of yield stability or yield risk based on simulated crop yield data? I would like to use a modelling approach and simulate grain yields...

23 February 2020 2,173 6 View

What is the best way to calculate the yield of an entire crop rotation including different kind of crops ?

What is the best way to calculate the yield of an entire crop rotation including different kind of crops and different yield levels ? For example the following cropping sequences: 1. sugar beet...

03 December 2019 10,017 11 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

Do we save MSMEs of the Nation ?

SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...

01 August 2024 5,562 2 View

What are the most effective strategies for integrating sustainability in your expertise?

What challenges do you think businesses or entities face in implementing these strategies? Thanks

28 July 2024 2,125 14 View

Has the Affordable Connectivity Program Helped You/Your Community? And Would Bulk Tablets on a Similar Program Help Your Business?

Do You Believe VP Harris Would Show Empathy to Tech Companies in Her Administration as POTUS? I ask in light of the fact that, the ACP was not re-funded this round. So many and businesses need...

24 July 2024 4,732 3 View

Can somebody share a diversity and inclusion questionnaire for the business context or military context?

Dear All, Can somebody share a diversity and inclusion questionnaire for the business context or military context. Thank you Anuradha

22 July 2024 1,320 4 View

What is the impact of leadership commitment towards business intelligence adoption?

How does leadership affect business intelligence adoption

17 July 2024 2,345 3 View

What are the job opportunities in USA for someone who has just obtained their Doctorate in Business Leadership?

I am searching for opportunities in USA. I recently obtained a Doctorate in Business Leadership. Kindly assist by sending links to jobs with visa sponsorship.

11 July 2024 9,948 1 View

In addition to traditional funding sources, are there any innovative funding models or industry partnerships that have been successful in supporting?

I am seeking funding to support a postdoctoral research position in the field of Business Administration with a specialization in Marketing.

08 July 2024 9,910 5 View

How much does a Business Analyst in JPMorgan Chase, Mumbai earn per annum?

I would like to know what is the yearly in-hand salary of someone with 2 years of experience working as a Business Analyst in JPMogan Chase in Mumbai?

05 July 2024 4,715 2 View