Seeing as vitamin D tests are very costly, I was wondering whether there are any cheaper methods to detect vitamin D in large-scale human blood samples in order to determine the effectiveness of maternal vitamin D supplementation.
There are many techniques for vi D determination including HPLC but the cheapest and easiest one is ELISA
Dear Kavitha, there are a lot of methods for VitD (25OH VitD and 1.25 (OH)2VitD) determination in serum/plasma samples. including Chromatography method,e.g. HPLC, LC-mass, immunoassay with differene lable, radioactive, enzyme, fluorosence, luminoscence,...But based on price and analysis speed, immunoassays are the best. You can find many valid companies which produce RIA or EIA assay kit for this purpose.
Hello, As noted above there are many ways to measure vitamin D levels. There are many considerations in addition to price that can help to determine the best test. These include the nature of your study (if vitamin D levels are your primary study outcome or are they just one of many factors you are interested in), if you are interested in other vitamin D metabolites than 25 and 1,25 and if you are just testing mothers or are interested in newborn levels as well. Some tests can diferentiated between C-3 epimers of d that others can not.
There is a quick, FREE test to see if you are very vitamin D deficient < 15 ng.
http://vitamindwiki.com/tiki-index.php?page_id=2095
We have used ELISA quite successfully but now other variants of ELISA that can also be used. But the basic principle is the same. ELISA has given us good results and we have used it in various diseases like asthma, T1DM, T2DM, PCO etc. If you really want to save money then buy good antibody commercially and make all other solutions your self. This way there will be a lot of cost cutting. Best of luck.
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