Charmm/GROMACS Residue 'ZN' not found in residue topology database?

I am trying to do some MD simulations of a protein with zinc ions. I am trying to use a charmm forcefield (I have tried 2017 and 2021) and Gromacs. I get an error when doing pdb2gmx Residue 'ZN'...

12 February 2021 9,367 2 View

How much does bead beating for cell lysis heat the samples (reg. pre-cooled adapters for the Qiagen Tissue Lyser II) ?

Hello, I would like to isolate total protein for phosphoproteomics using the Qiagen Tissue Lyser II (that we have in our department) for disruption of yeast lysis with glass beads. However,...

10 October 2019 8,861 3 View

Which method of euthanasia is most appropriate for preserving the redox state of proteins from whole organs?

I've sacrificed a small number of mice via isofluorane + CO2 and quickly took all major organs and flash froze them with liquid nitrogen. The goal is to preserve the redox state of proteins in...

18 September 2017 8,160 3 View

Does anyone have a copy of the revised Self-Perception Profile Questionnaire?

11 June 2017 2,969 3 View

How to implement Exhaustive Search Method for Multi-objective Optimization?

25 May 2016 6,101 4 View

Is there any research linking AIDA and Social Media?

I am currently researching on the impact of the social media on consumers using the AIDA funnel. I have looked for published research, but I have not been able to find any research relevant to my...

24 September 2014 5,480 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

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01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View