Hello,
I work in cancer immunotherapy particularly in CAR T-cells. I have leukaemia cell lines such as KO-52, ksaumi and THP-1. They express my target antigen at different levels. My CARs are also active, they kill ovarian tumour cell line expressing the same antigen but not leukemia cells. I am wondering what are the possible reasons for tumour cells resisting killing in vitro.
Thanks
Abed