Aside from M9 Minimal Media, what are other types of minimal media?

My partner and I are trying to culture bacteria. We are attempting to culture bacteria in minimal media using plastic as its carbon source. We would like to know an alternative in case we do not...

04 May 2018 8,411 3 View

What are possible ways of creating amorphous PET from non amorphous PET?

We currently have plastic bottles that are non amorphous. Is there a methodology available that could be done in order to transform these plastic bottles into amorphous form? Thank you

03 April 2018 8,457 0 View

What antifungal agent could be added to nutrient agar?

We are planning to grow bacteria on nutrient agar, but we would like to avoid the growth of fungi. We only want bacteria; thus, what antifungal agent could we add to the nutrient agar?

03 April 2018 3,863 14 View

Are there ways that a siderophore could be physically isolated from fungi?

My partner and I are conducting a study in siderophores in fungi. We would like to know if there would be any way that we would be able to isolate the siderophore from the fungi itself.

01 February 2018 5,119 8 View

What are the different types of species of fungi that has siderophore?

My partner and I are conducting a research on fungal siderophores. We would like to know what species of fungi contains siderophores.

01 February 2018 5,440 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View