To know more about the Dna cloning vectors
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please suggest the research paper related to the instrument is used in it.
16 July 2024 8,453 1 View
Is it possible to differentiate or calculate, the amount of ferromagnetic and antiferromagnetic contribution in a canted system? (Weakly frustrated canted antiferromagnetic system)
25 June 2024 6,210 0 View
In many papers, they have given the atomic position, lattice parameters, bond length, bond angle etc. values with (). what is the value of inside the bracket? If it is error bar how to find in...
13 June 2024 7,607 2 View
Menurut Bapak/Ibu, apakah editor jurnal lebih memilih Systematic Literature Review 10-20 tahun daripada yang menelaah 5 tahun? In your opinion (Mr and Ms), what do you think, journal editors...
26 May 2024 140 2 View
I am preparing a case study of a social work professional organisation which is non-existent now but had a glorious past. For this, I interviewed around 20 members of the association to understand...
18 May 2024 6,656 3 View
Machine Learning or AI Tools roleplay in the Automation of Software Testing
17 February 2024 159 0 View
In order to prepare conditioned media from primary cultured dental pulp cells, passage 5 is widely reported. May I have some scientific explanation despite of the inadequate quantity of the cell....
09 January 2024 898 2 View
Any harmful effects on the genetic material of microbes due to nitrate exposure. When nitrate is uptake by the bacterium, molecular mechanism involved how it is causing DNA or RNA damage?
03 December 2023 9,886 2 View
I noticed layer was formed during NK cell culture (initial day) of a cancer patient. Could it be platelet aggregation? What are the causes of layer formation in NK cell culture?
23 November 2023 6,674 0 View
Please share the GC-MS sample preparation of algal biomass. My research is focused and interested in lipid content should I go for whole biomass or extract lipid and then perform the FAME analysis...
14 November 2023 3,946 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
