I created fermentation media using 1%CMC, 0.25 gm K2HPO4, 0.125 gm KH2PO4

MgSO4.7H2O 0.05, NaCl 0.125 gm, CaCl2.2H2O 0.005 gm, FeSO4 0.0025 gm, 1gm Lactose in 250 ml

pH was set to 7.0

Incubation set to 37 C for 72 hr with agitation.

But "There is no apparent enzyme activity when measuring according to Ghose endocellulase assay"

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