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Questions related from Khaled Mahmoud
I created fermentation media using 1%CMC, 0.25 gm K2HPO4, 0.125 gm KH2PO4 MgSO4.7H2O 0.05, NaCl 0.125 gm, CaCl2.2H2O 0.005 gm, FeSO4 0.0025 gm, 1gm Lactose in 250 ml pH was set to 7.0 Incubation...
09 July 2025 3,912 3 View
I need help in calculating FPU using CMC as cellulose alternative where i missed the dilution step which is adding 20 ml distilled water as mentioned in CARBOXYMETHYL CELLULASE ASSAY FOR...
09 July 2025 1,848 0 View
I use Adney and Baker method https://permanent.fdlp.gov/lps94126/42628.pdf to calculate Cellulase enzyme FPU. but in section 9.3 they say "plot of glucose librated against the logarithm of enzyme...
16 January 2025 5,757 0 View
I have multiple biomass samples, i need to design an ionic liquid or deep eutectic solvents, for pretreatment Where to start I have already many literature reviews and papers but common thing is...
14 January 2025 9,492 4 View
What are suitable methods for measuring ethanol resulting from fermenting pretreated sugarcane bagasse? If possible provide a method not suggestion and kindly don't provide AI answers
14 January 2025 2,916 4 View
Do i know someone who used this method: Crowell, E.A. and Ough, C.S. (1979) A Modified Procedure for Alcohol Determination by Dichromate Oxidation. American Journal of Enology and Viticulture, 30,...
14 January 2025 9,786 1 View
If you want to design an Ionic Liquid or deep eutectic solvent for lignocellulosic biomass hydrolysis, where to start, any advice will be helpful.
03 November 2024 2,301 1 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
07 August 2024 1,302 1 View
I have produced Cellulase enzyme from A. Niger, the assay gave 100 FPU/ml now i want to add 30 FPU to SSF fermentation media that is contains 10 gm bagasse, at 25% cellulose the media is about...
07 August 2024 345 1 View