I currently use ammonium acetate but am looking to try a few other buffers. So far on my to buy list I have; ammonium formate, ammonium bicarbonate and 4-methylmorpholine. Can anyone please recommend any others I should be trying please? Thank you.
In protein analysis we use milliQ water/Acetonitrile with 0.1% TFA (but TFA tends to suppress the MS signal, so you should use 0.05% or less).
I think you should try 0.1% Formic Acid. It will probably give you slightly lower resolution of peaks but a stronger MS signal.
Hi Aimee, here are lists of volatile buffers, I hope it helps.
http://www.nestgrp.com/protocols/trng/buffer.shtml
http://wolfson.huji.ac.il/purification/buffers/Volatile_buffer.html
https://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf
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