I've always used abd as load control for western blot normalization, and use Ponceau S stain as a transference control (along with keeping an image - a picture taken by my cellphone - for supplementary paper material submission). My question is:
Can I use this picture to quantify total protein? Or there is a specific way/equipment to image the membrane stained with Ponceau S to quantify total protein?
Hi Aimée Obolari Durço
Depending on the quality of the image, it may be possible to use your Ponceau image along with our Phoretix 1D software - https://totallab.com/products/phoretix-1d/ to create a multiplex image, then use the Ponceau channel for total protein normalisation.
I've done a tutorial video on YouTube on how to normalise Western Blots within our software which you can find here - https://youtu.be/H05aRpw8Wdg?si=LJNbvUcoakrFBmHw
Hope this helps.
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