I am interested in culturing murine bone marrow cells ex vivo in order to study the effects of a drug on B cell development. I am wondering if anyone can direct me to papers reporting methods for doing so. Thanks.
It looks like you do not want to differentiate the bone marrow stem cells into any one kind of lineage, but want to study the effects of the drug on B cell development. This paper addresses the need for bone marrow mesenchymal cells for growth of bone marrow cells (successful). However they talk about B cells that are already committed to the lineage (which I feel would address the question you ask)