If anyone has worked with a cell line like this, could you recommend culture techniques? What medium to use, how often to passage, etc.
RPMI 1640. The little secret is the mercaptoethanol.
Check the paper.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC84281/
Good luck with your experiments!!!
Use RPMI 1640+ L glutamine+ Mercaptoethanol
Use 10 to the power 6 cells for 48 to 72 hrs
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