As I am going to start diagnosis of several diseases by PCR techniques at our hospital, any one can help me the list of diseases can be diagnose and probable solution (Chemical, primers and ingredients) to detect those diseases.
The help will definitely enrich the scrutiny of various diseases in a quick process through PCR techniques.
waiting for your valuable help.
with regards
Gradient PCR - using different annealing temperature to optimize the Primer and get amplification (only able to say YES/NO - infected or not)
Realtime PCR - Quantify the (Patient sample) template concentration (estimate the Infection level - severity of the disease)
Viral diseases, Bacterial infection
Thank you Ellango
Need to know the diseases to be detected or quantify from these technique.
with regards
I think you could diagnose if you know pathogen specific sequence .
for more knowing disease names go through this paper:
"Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing"
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1360278/
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