What are Abiraterone, long term side effects in Ca. prostate ?

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Using Ficoll-400 to separate viable cells from dead cells in cell culture suspensions?

Will someone have a protocol or reference on using Ficoll-400 to separate viable vs dead cells in suspension cultures? I am culturing Jukrat cells but will also be culturing DU4475s soon. Thanks

13 February 2024 1,449 1 View

Can we use gut microbiome whole cell mix (MSA-2006™) in place of fecal sample for in vitro batch fermentation experiments?

Presently we are developing anerobic batch fermentation for understanding the impact of plant isolates at gut microbiome level. Fecal samples are dominantly used for acquiring health gut...

09 July 2023 6,962 0 View

Please suggest the role of low and high molecular weight Na-alginate powder for the encapsulation of phenolic acids ?

Na-alginate powder available in two forms (low and high molecular weight) and both are available for the preparation of Ca-alginate beads. I want to know that how it will affect the encapsulation...

29 January 2019 4,040 3 View

What are the different reasons behind higher phenolic content but lower antioxidant activity ?

Hello everyone, I have a query regarding ''the possible reasons behind the higher phenolic content but lower antioxidant activity''. I used the raw bamboo shoots and it showed good amount of...

11 June 2018 2,978 18 View

How to calculate the phenolic/flavonoid content in oil/water nano emulsion?

I usually centrifuge the nano-emulsion, take out the aquaous media and add same vol. of methanol followed by vortex and ultrasonication. A clear supernatant sol. of methanol was obtained after...

05 February 2018 1,871 4 View

How to make the continuous suppy of N2 gas during the bound phenolic extraction?

It is mentioned that N2 gas is provided during the alkaline hydrolysis of plant matrix or residual part for the extraction of phenols. I made some arrangements using N2 gas balloons but there is a...

21 February 2017 9,735 1 View

Is there any database for phenolics?

Phenolic quantification with a absolute precision MALDI-TOF is performed. Is there any database exist by which we can compare our ms/ms data? I am already aware about the phenol explorer. But I...

28 December 2016 3,957 6 View

What is the possible reasons behind the no zone of inhibition in 70% ethanolic extract?

A 70% ethanolic plant extract dissolved in 70% ethanol was prepared for anti-microbial activity (zone of inhibition) with 1x10 pow.7 cells per mL of klebsiella. But no zone of inhibition was...

21 December 2016 537 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View

MTT assay - 6 well plate?

Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...

13 July 2024 9,783 1 View

How to convince decision makers to adjust the legislation to allow opening of drug consumption rooms?

In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...

08 July 2024 8,903 1 View

Is the use of microcrystalline cellulose MCC 102 in direct compression technology justified, given the low flowability of this excipient?

Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...

08 July 2024 2,807 0 View

What are the trending topic to writ e a review article on it?

The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field

02 July 2024 4,031 3 View