14 February 2024 1 1K Report

Will someone have a protocol or reference on using Ficoll-400 to separate viable vs dead cells in suspension cultures? I am culturing Jukrat cells but will also be culturing DU4475s soon. Thanks

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Can we use gut microbiome whole cell mix (MSA-2006™) in place of fecal sample for in vitro batch fermentation experiments?

Presently we are developing anerobic batch fermentation for understanding the impact of plant isolates at gut microbiome level. Fecal samples are dominantly used for acquiring health gut...

09 July 2023 6,962 0 View

What are Abiraterone, long term side effects in Ca. prostate ?

What are the long term side effects of Abiraterone drug used in Ca. prostate in combination with androgen deprivation therapy?

07 September 2021 8,056 3 View

How to properly freeze and thaw THP1 cells?

I have been trying to freeze and thaw THP1 cells for quite some time now. I have been through different protocols suggested by colleagues as well as in different forums, but so far nothing has...

23 December 2019 7,613 9 View

Please suggest the role of low and high molecular weight Na-alginate powder for the encapsulation of phenolic acids ?

Na-alginate powder available in two forms (low and high molecular weight) and both are available for the preparation of Ca-alginate beads. I want to know that how it will affect the encapsulation...

29 January 2019 4,040 3 View

What are the different reasons behind higher phenolic content but lower antioxidant activity ?

Hello everyone, I have a query regarding ''the possible reasons behind the higher phenolic content but lower antioxidant activity''. I used the raw bamboo shoots and it showed good amount of...

11 June 2018 2,978 18 View

How to calculate the phenolic/flavonoid content in oil/water nano emulsion?

I usually centrifuge the nano-emulsion, take out the aquaous media and add same vol. of methanol followed by vortex and ultrasonication. A clear supernatant sol. of methanol was obtained after...

05 February 2018 1,871 4 View

How to make the continuous suppy of N2 gas during the bound phenolic extraction?

It is mentioned that N2 gas is provided during the alkaline hydrolysis of plant matrix or residual part for the extraction of phenols. I made some arrangements using N2 gas balloons but there is a...

21 February 2017 9,735 1 View

Is there any database for phenolics?

Phenolic quantification with a absolute precision MALDI-TOF is performed. Is there any database exist by which we can compare our ms/ms data? I am already aware about the phenol explorer. But I...

28 December 2016 3,957 6 View

What is the possible reasons behind the no zone of inhibition in 70% ethanolic extract?

A 70% ethanolic plant extract dissolved in 70% ethanol was prepared for anti-microbial activity (zone of inhibition) with 1x10 pow.7 cells per mL of klebsiella. But no zone of inhibition was...

21 December 2016 537 4 View

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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

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