A 70% ethanolic plant extract dissolved in 70% ethanol was prepared for anti-microbial activity (zone of inhibition) with 1x10 pow.7 cells per mL of klebsiella. But no zone of inhibition was found? What is the possible reasons behind it ?
There are several reasons for this. It is possible that the extract really has no antibacterial activity. The concentration of the extract might also be a reason, it might be too low to elicit antibacterial activity. It is also possible that because this is still a crude extract, the bioactive compound is masked by other compounds present in the extract. Hope this helps.
There are several reasons for this. It is possible that the extract really has no antibacterial activity. The concentration of the extract might also be a reason, it might be too low to elicit antibacterial activity. It is also possible that because this is still a crude extract, the bioactive compound is masked by other compounds present in the extract. Hope this helps.
you can see this article
Antimicrobial Activity of Crude Extracts of Some Plant Leaves
May be because the concentration very low. You must concentrate the extract, or there's no activity to the type of bacteria. Change the type of bacteria.
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