I already have a T.muris strain, but mice infection required a previous prednisone treatment. This is an inconvenient if you look at a immune induced by a pan-nematode antigen.
Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...
11 July 2024 6,847 0 View
Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...
18 April 2024 5,671 3 View
Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...
10 March 2024 4,156 3 View
Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...
22 February 2024 8,052 2 View
Hi, Does anyone know about a public transcriptomic dataset (RNAseq, microarray) of Arabidopsis (or other plants) treated with Staurosporine? I checked in GEO or SRA, but I couldn't find any....
11 February 2024 8,621 0 View
I have been performing titrations of a known concentration of hydrogen peroxide using 0.01N potassium permanganate and consistently requiring more potassium permanganate than what is estimated to...
07 February 2024 446 3 View
I am conducting a sequential heat transfer-stress analysis for a composite wall consisting of steel plates and a concrete core, similar to concrete-filled tubes or CFTs. My analysis involves...
06 February 2024 1,525 6 View
I'm running XF T Cell persistence assay in a mini Seahorse. I used to not have problems until I start getting some negatives values in OCR measurements, and also errors with both background wells,...
12 January 2024 4,455 0 View
Good morning, colleague My name is Víctor Hugo Fernández Bedoya, and I am a professor at the National University of San Marcos (Universidad Nacional Mayor de San Marcos), located in Lima, Peru. I...
08 January 2024 1,652 0 View
Hello everyone, I need advice on improving the quality of my coronal slices obtained from PFA-fixed mouse brains using a cryostat. As You can see in the attached image, slices are horrible! Any...
04 January 2024 6,071 2 View
Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore
04 June 2024 5,688 2 View
The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...
12 March 2024 3,926 0 View
Hi, We are trying to isolate rat MSCs from Fischer F455 rats. So far we tried a femur flush protocol to flush the bone marrow into a petri dish. However, we are wondering if we should do...
12 July 2023 4,684 0 View
Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...
17 April 2023 3,999 0 View
In the field of biosciences we often get in contact with the questions: Are viruses alive, a form of life? Is fire a form of life? Stars also burn fuel and stay stable until their fuel is burned...
16 April 2023 7,233 1 View
I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...
12 January 2023 4,248 2 View
The protein was extracted from mouse tissue(brain),marker is Thermo 26619. The ladder was about in 25kd.And the antibody is BD Bioscience#asyn antibody. I'm not sure if it was α-synuclein.
08 December 2022 9,772 4 View
I've grown my influenza straind in MDCK cells and have isolated them. Now I have to sequence my virus, my lab uses Eton Biosciences. Do I send in primers for each gene? Do I extract RNA from the...
18 May 2022 2,592 0 View
The reporter cell line has been cultured following the suppliers recommendation (see below): Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Thermo Fisher, #11095098) supplemented with 10% FBS...
09 January 2022 3,186 0 View
Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...
05 October 2020 1,928 2 View