when we apply the PRP on the wound the GFs will be released, why?
Calcium activates platelets. There is Calcium in blood and fluid that leak from wounds. Putting it on a wound will lead to activation. Also injecting into a wound will lead to activation and release of the GF's.
PRP can be activated in various ways, for example with calcium chloride, autologous thrombin, a combination of the 2 mentioned and also with certain collagens (specifically collagen type I).
In wounds, platelets are activated spontaneously after exposure to the native collagen present in the connective tissues. However, collagen is a weak activator. Adding one or more of the others (CaCl2/ Thrombin/ combination) stimulates a more rapid and/ or sustained release of GFs. As to your final question, PRP can certainly be activated to release GFs in a test tube provided that one or more of the "natural" activating factors present in all wounds (such as collagen, calcium etc) is present.
There are two forms of PRP activation; Endogenous and Exogenous. In the former, PRP is activated physiologically in the body when it comes in contact with one of its activators. Exogenous activation (here is in the test tube) can be either by physical methods such as freeze thaw cycle or the adding substances such as CaCl2 or thrombin. Either way there should be an activator for GF release.
the platelet-rich plasma must be prepared in a test tube that has anticoagulant EDTA which keeps the PRP safe.
when we apply this PRP on the wound or tissue where the coagulation factors exist, the PRP will be breakdown and will release the growth factor
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